![]() More than half of the bands for Biorad product disappears or becomes very faint. In comparison, Thermo’s 10 KD band is almost completely disappeared, and the two red bands is very faint. After overnight washing, only 25K and 20K of our marker are weakened, and the rest of the bands are still very clear. After 3 hours, all bands of our products are very clear while the brightness of Thermo’s 10 kD band has been extremely weak and Biorad’s 25K band has completely disappeared, and its 10 KD band is also extremely weak. In a controlled experiment, our marker and ones of Thermo and Biorad are subjected to 3 hours to overnight washing with TBST washing buffer. It can take as short as 3 hours or even overnight. The membrane (PVDF or NC) after transferring during western blot experiment needs to undergo blocking, primary and secondary antibody incubation, and a multi-step washing process in between. Note that the red band in Thermo’s product cannot be scanned by NIR fluorescence either. All bands except orange ones are well compatible with near-infrared (NIR) fluorescent system. The images are produced from a single controlled experiment. Dyes only fade slightly and very little or almost no degradation of the marker proteins occurs even under the extreme temperature (37 ☌) ) for one month (lanes 2 and 4). The results for a comparative study under two different temperatures (-20☌ and 37☌) are shown in this image. All the markers are subject to strict stability QC before release. Our Prestained Protein Markers are very stable. Even more, Thermo has many inconsistencies in the molecular weight between its prestained and non-prestained protein markers. Biorad ladder has two inaccurate bands of 37K and 75K while Thermo prestained products have inconsistent 35K and 25K. Biorad and Thermo (Compare lanes 3, 4 and 5 with ours lane 6). ![]() MW Precision NewEast Biosciences Prestained Protein Marker is as accurate as the current non-prestained ladders on the market (Compare lanes 1 and 2 with ours lane 6), but has superior MW precision in comparison with the major market suppliers, ie. Highlights – Broad MW range (10-250 kD)Ģ. ![]() Ready to use, with no heating, dilution or additional reducing agent required.ġ. The protein markers are highly stable with minimal band broadening during storage. Markers are confirmed in Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. Red and green bandsĪt 25 kD and 70 kD and provide easy references for molecular weight identification. # : 22301ĭescription: Tri‐Color Prestained Protein Marker – RBGĭual-Color Prestained Protein Markers are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Some experiments require only a qualitative answer, but properly designed and executed western blot experiments can also provide quantitative data on relative protein expression between samples.Tri‐Color Prestained Protein Marker – RBG Cat. Understanding basic imaging concepts such as sensitivity, resolution, and sources of background noise can help you maximize image data quality.Īn image of a western blot is rich with information. In addition to detection of specific proteins, the total protein in a sample can also be visualized with total protein stains or newer technologies that eliminate the need for staining and destaining.Īccurate imaging of your western blot is crucial for capturing blot data for downstream analysis. Efficient protein transfer is required for maximum western blot sensitivity.Ī good, clean western relies on the specificity and sensitivity of your antibodies. Separated proteins are transferred from the gel to a membrane where they are immobilized. ![]() Good sample preparation techniques ensure proteins remain undamaged for downstream analysis.Įlectrophoresis separates the proteins in the sample and provides molecular weight data for detected proteins during subsequent detection. Cells containing your protein of interest must be lysed completely to ensure a high yield while removing non-protein components of cells. ![]()
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